Figure 4 Changes in chromosomal accessibility during aging. (A) Heatmaps showing scaled expression of discriminative gene sets for each cell type and cell subset. Color scheme is based on z-score distribution from −1.5 (purple) to 1.5 (yellow). (B) t-SNE projections of PBMCs derived from scATAC-seq data. (C) t-SNE plots segregated by YA and AA groups. (D) UpSet plot showing the integrated comparative analysis of upregulated differentially expressed transcription factors (DETs) in major immune cell populations between YA and AA groups. Upregulated DETs: upregulated in AA, downregulated in YA. The count showing the number of DETs. (E) UpSet plot showing the integrative comparative analysis of downregulated DETs in major immune cell populations between YA and AA groups. Downregulated DETs: upregulated in YA, downregulated in AA. The count showing the number of DETs. (F) Venn diagram showing integrated comparative analysis of upregulated DETs in CD4+ T cells between YA and AA groups. Upregulated DETs: upregulated in AA, downregulated in YA. The count showing the number of DETs. (G) Venn diagram showing integrated comparative analysis of upregulated DETs in CD8+ T cells between YA and AA groups. Upregulated DETs: upregulated in AA, downregulated in YA. The count showing the number of DETs. (H) Venn diagram showing integrated comparative analysis of upregulated DETs in NK cells between YA and AA groups. Upregulated DETs: upregulated in AA, downregulated in YA. The count showing the number of DETs. (I) Venn diagram showing integrated comparative analysis of upregulated DETs in B cells (top) and monocytes (bottom) between YA and AA groups. Upregulated DETs: upregulated in AA, downregulated in YA. The count showing the number of DETs. (J) Mean scATAC-seq coverage at FOSL2 loci in CD8+ T cells. (K) Mean scATAC-seq coverage at NFATC2 loci in CD8+ T cells. (L) Mean scATAC-seq coverage at CDKN2B loci in B cells. (M) Mean scATAC-seq coverage at SIRT7 loci in NK1 cells. (N) Mean scATAC-seq coverage at GLI2 loci in CD4 Naive cells. (O) Mean scATAC-seq coverage at IFNG loci in CD8 Naive cells. (P) Mean scATAC-seq coverage at DUSP5 loci in CD8 Memory cells. (Q) Mean scATAC-seq coverage at PDCD1 loci in NK3 cells