We next investigated how the UMAP Components were associated with individual immune features (tables S5 and S6). UMAP Component 1 captured immune features, including the relative loss of CD4 and CD8 T cells and increase in nonB:nonT cells (Fig. 6G). PB also associated with Component 1 (Fig. 6G). Other individual B cell features were differentially captured by UMAP Component 1 and 2. Component 1 contained a signal for T-bet+ PB populations (table S5) whereas Component 2 was enriched for T-bet+ memory B cells and CD138+ PB populations (table S6). Activated HLA-DR+CD38+ and KI67+ CD4 and CD8 T cells had contributions to both Component 1 and Component 2, with these features residing in the upper right corner of the UMAP plot (Fig. 6, G and H, and fig. S8, A to D). In contrast, T-bet+ non-naïve CD8 T cells were strongly associated with Component 2 whereas T-bet+ non-naïve CD4 T cells were also linked to Component 1 (Fig. 6G and tables S5 and S6). Eomes+ CD8 or CD4 T cells were both associated with Component 2 and negatively associated with Component 1 (Fig. 6G and tables S5 and S6).