Single-cell RNAseq analysis of coronavirus receptors in adipose tissue.
UMAP-based dimensionality reduction plot denoting cell types (A, C, E) and corresponding expression levels of Ace2, Dpp4, Anpep and Tmprss2 (B, D, F) from multiple studies: (A–B) adipose stromovascular fraction from mice treated with PBS or a beta-3 adrenergic agonist (Rajbhandari and Arneson, 2019), (C–D) murine adipose stromovascular fraction from Tabula Muris (Tabula Muris Consortium et al., 2018), and (E–F) adipose tissue from mice subjected to 24 hr of cold shock and processed for single nucleus RNA-sequencing (Rajbhandari and Arneson, 2019). (G–H) Identification of adipocytes as the most likely ACE2-expressing cell type in adipose tissue based on literature associations. The query shown is from the nferX Signals platform and effectively extracts any textual fragments which contain [‘ACE2’ or its gene synonyms] AND [displayed terms related to gene expression/protein detection] AND [‘adipose’ OR 'fat']. Among these textual fragments, we extract the tokens which represent cell types and calculate enrichments of each cell type among these fragments, normalized by the number of occurrences of this cell type elsewhere in the literature.