Twenty one of the 508 RSs from Pteropus spp. bats screened were tested positive for the BtCoV (Table I). These positive bats belonged to Kerala (n=12), Himachal Pradesh (n=2), Puducherry (n=6) and Tamil Nadu (n=1). The TS specimens of the same bats were tested negative for BtCoV. The TS specimens of RS-negative (n=42) bats were also screened and found to be negative (Table I). A total of 25 bats from both the species were found positive.