Detection of bat coronavirus using RdRp gene RT-PCR: Four of the 78 RS of Rousettus spp. bats screened for the BtCoV were found positive. All the positive RS samples belonged to Kerala State. Intestinal specimens of two bats were also found to be positive for the BtCoV. One bat (MCL-19-Bat-606), from Kerala, was tested positive in both the intestinal specimen and the RS. The second bat (MCL-20-Bat-76), from Karnataka, was tested positive only in the intestinal specimen. Altogether, five Rousettus spp. bats were positive for the BtCoV. All TS specimens from Rousettus spp. were found negative for BtCoV (Table I).