2  Methods
From the NCBI virus database, all the protein sequences of 3617 SARS-CoV2 genomes were fetched. Then the amino acid sequences of envelope protein of SARS-CoV2 are exported in fasta format using file operations through Matlab. These sequences (fasta formatted) are blasted using Clustal-Omega and found the mismatched and from their mutations and their associated positions were detected [13].