In healthy subjects, different daily doses of EPA and DHA up 1800 mg, administered up to 5 months, showed no significant effects on the CPR, IL-6, and TNF-α (Asztalos et al., 2016; Flock et al., 2014; Muldoon et al., 2016). A comparable conclusion was drawn by other authors. According to Rangel-Huerta's meta-analysis, consumption of 900 mg–2000 mg n-3 PUFAs does not change inflammatory biomarkers in healthy subjects (Rangel-Huerta et al., 2012). On the other hand, doses between 1250 and 2400 mg n-3 PUFA for 4 months lowered inflammation in sedentary and overweight middle-aged and older adults (Kiecolt-Glaser et al., 2012). Lp-PLA2, another anti-inflammatory marker was significantly reduced by a high dose of EPA (1800 mg) but not by DHA (Asztalos et al., 2016). Interestingly, only DHA modified the lipid profile by decreasing postprandial triglyceride concentrations and significantly increasing low-density lipoprotein cholesterol, with no significant changes in inflammatory biomarkers (Asztalos et al., 2016). In elderly subjects, daily supplementation with 2500 mg EPA and DHA, for 8 weeks, significantly reduced the plasma levels of fatty acids, IL-1β, IL-6, and TNF-α (Tan et al., 2018). Similarly, in obese patients who received different doses of combined n-3 PUFAs (380–1290 mg DHA and 360–460 mg EPA) for 2–3 months, the intervention reduced the expression of proinflammatory genes in adipocytes and systemic inflammatory markers sVCAM-1, CRP, IL-6, and TNF-α (Itariu et al., 2012; Polus et al., 2016). Furthermore, other relevant metabolic findings connected with n-3 PUFA treatment were reported, such as decreasing fasting triglycerides and insulin (Allaire et al., 2016; Polus et al., 2016) or decreasing fasting blood glucose in obese diabetics (Ellulu et al., 2016). Partially, these results are in line with the modest reduction in waist circumference and body-weight found in a meta-analysis (Bender et al., 2014). The authors indicated that the effect regarding waist circumference produced by fish intake or fish oil supplementation and might be greater in men than in women. The beneficial effect in overweight and obese adults concerning waist circumference and triglyceridemia was confirmed by two other meta-analyses (Du et al., 2015; Zhang et al., 2017).