High-saturated fat diet (HFD) male C57BL/6J mice 3-week treatment:➢ standard chow
➢ HFD (60% fat 20% protein, and 20% carbohydrates)
➢ Very HFD (80% fat 16% protein, and 4% carbohydrates) HFD: ↑ leptin, IL-1β Andre et al. (2017)