Figure 7  Dl-3-n-butylphthalide (NBP) upregulates AMPK/SIRT1 signaling after 2VO. (A) Western blot analysis of the expressions of p-AMPK and SIRT1 (n = 6 in each group). (B) Quantitative analysis of protein levels of p-AMPK. (C) Quantitative analysis of protein levels of SIRT1. (D) Quantitative analysis of mRNA levels of SIRT1 (n = 6 in each group). β-actin was used as an internal control. *p < 0.05, **p < 0.01, ***p < 0.001, the model group vs. the sham group; #p < 0.05, ##p < 0.01, ###p < 0.001, the NBP60 group or NBP120 group vs. the model group; $p < 0.05, $$$p < 0.001, the NBP60 group vs. NBP120 group; and &p < 0.05, &&p < 0.01, the model group sacrificed at 2 weeks vs. the model group sacrificed at 4 weeks. Values are expressed as mean ± SD. p-AMPK, phosphorylated AMP-activated protein kinase. SIRT1, silent mating type information regulation 2 homolog 1.