ing assay as described above. n.i: no inhibition detected at 50 μM; n.d: not determined due to inhibition lower than 50% at 50 μM.

2.5  Thermal shift DSF assays for SARS-CoV nsp14
SARS-CoV nsp14-inhibitor interactions were further investigated by monitoring the thermal stability of the protein using differential scanning fluorimetry (DSF) (Supporting Information, Fig. S3) [34]. The change in thermal stability of SARS-CoV nsp14 was monitored in