ir specificity (Table 1 ).
Table 1 Screening for inhibitory activity of sinefungin and compounds 1–16 at 50 μM on N7-MTases.
Compounds Percentage of inhibition at 50 μM (%)a
SARS-CoV nsp14 Vaccinia virusD1-D12 hRNMT
Sinefungin 98.3 ± 0.2 99.8 ± 0.1 99.8 ± 0.2
1 31.0 ± 6.8 20.3 ± 0.8 35.2 ± 4.9
2 72.0 ± 1.2 85.8 ± 2.5 77.4 ± 1.2
3 30.6 ± 9.3 32.1 ± 2.4 33.2 ± 4.3
4 13.1 ± 13.3 53.2 ± 2.6 12.2 ± 2.1
5 n.i n.i 27.5 ± 6.6
6 38.4 ± 11.7 11.6 ± 7.1 23.1 ± 9.7
7 n.i 69.2 ± 1.9 32.8 ± 16.1
8 43.0 ± 4.0 n.i n.i
9 88.6 ± 1.3 49.8 ± 3.2 66.0 ± 6.1
10 96.6 ± 0.9 4.6 ± 0.3 31.8 ± 3.3
11 47.6 ± 2.8 5.3 ± 4.3 44.2 ± 8.5
1