An immunohistochemical investigation demonstrated microscopic lesions suggesting broncho-interstitial pneumonia in the lung tissues of four severely sick piglets at a swine farm located in Parana province in February 2011. The A(H1N1)pdm09 viruses were isolated from two piglets. Additionally, a novel reassortant H1N2 virus was also recovered [327]. One more investigation identified that A(H1N1)pdm09 virus was the most prevalent IAV subtype in sows. The co-infections of sows with A(H1N1)pdm09, H1N2, or H3N2 subtypes were also documented in Rio Grande do Sul province. These findings were noteworthy because the coinfections may trigger reassortments and thus may facilitate emergence of novel strains of IAV [328]. Later two more H1N2 viruses were isolated and characterized from swine in Rio Grande do Sul province during 2013. The sequences of both the isolates had high nucleotide similarity to each other in different genome segments in the range of 98.9% to 100% which suggested a common source of origin of both isolates [329].