The oral fluid samples collected from 25 neonatal piglets at four Oklahoma based swine farms during May–August 2014 [297] were found infected with different IAV subtypes including H1, N1, H3, and N2. This study supported the use of swine oral fluid samples in IAV diagnostics [285]. The swine oral fluid samples were also collected in North and South Carolina during June to August 2014 using the cotton rope hanging method [298]. In this method of sampling, swine are encouraged to chew the rope, as a result, saliva accumulates on the rope which is later squeezed to collect the sample aseptically. One of the benefits of this method of sampling is that each sample does not represent an individual swine but rather represents multiple swine that chewed the rope while hanging inside the pen [298]. Another benefit of this sampling method is that swine oral samples may contain contaminants like feed and feces but this method minimizes the chances of such contaminations in the sample [299].