Second report of IAV in Australian swine appeared on a Queensland farm in August 2009 when a veterinarian observed elevated temperature, coughing and loss of appetite in swine. Simultaneously, two of the staff members on the farm exhibited influenza-like symptoms and hence were sampled for diagnostic testing using nasal swabs. Interestingly, both the staff members and four of the swine were found positive for the A(H1N1)pdm09 virus. Sequencing identified that the HA gene of A(H1N1)pdm09 virus retrieved from a staff member was identical to the virus retrieved from the swine which suggested transmission of A(H1N1)pdm09 virus between swine and human [176].