10.  Lung homogenate  RNA extraction, real-time RT-PCR, multiplex RT-PCR, single step RT-PCR, virus isolation (MDCK cells/Caco-2 cells/SPF chicken eggs), Sanger sequencing, membrane enzyme immunoassay, HI assay  H1N1, H1N2, H3N2, Reassortant H1N2, A(H1N1)pdm09  [130,213,218,292]