Nasal swab  RNA extraction, real-time RT-PCR, reverse transcription PCR, multiplex RT-PCR, ligation, partial/whole genome Sanger sequencing, Next-generation sequencing, phylogenetic analysis, virus isolation (MDCK cells/Caco-2 cells/HRT18 cells/SPF chicken eggs), transmission electron microscopy, HI assay, NI assay, ultracentrifugation  H1N1, H1N2,H3N2, H4N6, A(H1N1)pdm09, Reassortant H1N1, Reassortant A(H1N1)pdm09, Triple-reassortant H3N2, H1N7, H5N2, IBV, IDV  [5,37,43,45,46,61,74,88,137,157,171,176,188,191,199,207,215,219,220,221,241,243,248,250,263,289,290,301,306,307,326,334]