Table III  Spike protein peptides capable of binding to major histocompatibility complex (MHC) class I predicted using NetCTL server
Peptide  Vaxijen  Interferon (IFN)-γ response  CTLPred Score (ANN/SVM)  MHC restriction
89-GVYFASTEK-97  0.711  Positive (1)  0.58/0.986  HLA-A*1101, HLA-A3, HLA-A*3101, HLA-A68.1, HLA-B*2705
166-FEYVSQPFL-174  0.632  Positive (0.087)  0.65/0.184  HLA-A2, HLA-A*0201, HLA-A*0205, HLA-A2.1, HLA-B*2702, HLA-B*2705, HLA-B*3701, HLA-B40, HLA-B*4403, HLA-B*5301, HLA-B*5401, HLA-B*51, HLA-B60, HLA-B61, HLA-Cw*0301, H2-Kb, H2-Kk,
256-WTAGAAAYY-264  0.630  Positive (0.576)  0.82/0.544  HLA-A1, HLA-B*2702, HLA-B*3501, HLA-B*4403, HLA-B*5301, HLA-B*5401, HLA-B*51, HLA-B*5801, HLA-B62, HLA-Cw*0702
348-VYAWNRKRI-356  0.500  Positive (0.499)  0.93/0.497  HLA-A24, HLA-B*5101, HLA-B*5102, HLA-B*5103, HLA-B*51, HLA-Cw*0401, H2-Db, H2-Kd, H2-Kk
503-YQPYRVVVL-511  0.596  Positive (0.292)  0.40/0.596  HLA-A*0201, HLA-A*0205, HLA-A24, HLA-B14, HLA-B*2702, HLA-B*2705, HLA-B*3902, HLA-B*5201, HLA-B*5301, HLA-B*5401, HLA-B*51, HLA-B60, HLA-B62, HLA-B7, HLA-B8, HLA-Cw*0401, HLA-Cw*0602, H2-Dd, H2-Kb, H2-Ld
510-VLSFELLHA-518  1.077  Positive (0.268)  0.86/0.276  HLA-A*0201, HLA-A*0205, HLA-A3, HLA-B*5301, HLA-B*51, HLA-B62
825-TLADAGFIK-833  0.578  Positive (0.014)  0.75/0.992  HLA-A1, HLA-A*1101, HLA-A3, HLA-A*3101, HLA-A68.1, HLA-A20, HLA-B*2705
1058-VVFLHVTYV-1066  1.512  Positive (1)  0.77/0.779  HLA-A2, HLA-A*0201, HLA-A*0205, HLA-A68.1, HLA-A2.1, HLA-B14, HLA-B*5101, HLA-B*5102, HLA-B*5103, HLA-B*5201, HLA-B*5301, HLA-B*5401, HLA-B*51
1210-WPWYIWLGF-1218  1.495  Positive (0.221)  0.68/0.0695  HLA-B*2702, HLA-B*2705, HLA-B*3501, HLA-B*3801, HLA-B*5101, HLA-B*5102, HLA-B*5201, HLA-B*5301, HLA-B*5401, HLA-B*51, HLA-B*5801, HLA-B62, HLA-B*0702, HLA-Cw*0401, HLA-Cw*0702.H2-Ld
Threshold of >0.7 nM was used for increased specificity of the prediction. The peptides were reconfirmed using CTLPred server using default parameters. The peptides that were classified as epitopes were further checked for their antigenicity score using the VaxiJen v2.0 tool