3.5  Evaluation of Physicochemical Properties
Several different physicochemical properties of the MEPVC were deduced from its sequence. The vaccine construct is 189 amino acids long with total number of 2984 atoms. The molecular weight of the construct is ideal i.e. 21.36 kD as small size construct is easy to handle and purify during experimental evaluation. The construct has instability index value of 35.43, signifying its high stability. The aliphatic index computed for the construct is 79.68, reflecting high thermostability. The estimated half-life in mammals, yeast, and Escherichia coli is 30 hours, > 20 hours, and > 10 hours, respectively. The Grand average of hydropathicity (GRAVY) score is -0.315 which highlights hydrophilic nature of the construct. The theoretical pI is 6.10 pointing to construct slightly acidic nature.