Individuals in the designated hospital were required to adhere to the regulations imposed in different areas, and crossing of those different areas was strictly prohibited. The results showed that application of physical barriers combined with behavioral management can effectively prevent the spread of SARS-CoV-2 in the designated hospital. Two positive samples taken from the living quarters indicated the importance of more careful and thorough environmental cleaning and disinfection in this area. The positive rates in areas such as general isolation ward 1, general isolation ward 3, general isolation ward 6, ICU, emergency room, and fever clinic in public area were, on average, higher. In our study, SARS-CoV-2 RNA was detected from the environmental surfaces frequently touched by patients and healthcare workers. The top 5 sites with a positive infection rate were beepers, water machine buttons, elevator buttons, computer mouses, telephones, and keyboards. Special emphasis should be placed on the cleaning and disinfection of the crucial parts of key departments, and intensive disinfection should be instituted and done by professional nurses.