In Table 8 , ProtParam analysis further reveals the stability of the considered epitopes and final revelation of one epitope ITLCFTLKR is screened out. This particular Epitope exhibits an instability index of 35.68, with a grand average of hydropathicity (GRAVY) calculated was 0.844, and the estimated half-life for this peptide was determined to be 20 h for mammalian reticulocytes.
Table 8 ProtParam analysis for selected epitopes.
Selected Epitope GRAVY Score Instability Index (Indication) Estimated Half-Life(Mammalian reticulocytes) Theoretical pI Aliphatic Index
ITLCFTLKR 0.844 35.68(Stable) 20 Hours 9.51 130.00
VYQLRARSV −0.067 70.73(Unstable) 100 Hours 10.83 118.89