REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
CD14-FITC (RMO52 clone) Beckman Coulter Cat#IM0645U; RRID: AB_130992
CD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226
HLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962
IgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963
IgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964
CD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965
CD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966
CD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965
CD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967
Quantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137
Mouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442
Chemicals, Peptides, and Recombinant Proteins
VersaLyse Lysing Solution Beckman Coulter A09777
Fixative Solution Beckman Coulter A07800
eBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51
IntraPrep Permeabilization Reagent Beckman Coulter A07802
RPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496
Lymphosep, Lymphocyte Separation Media Biowest L0560
PBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615
FBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615
Gentamycin Sulfate BioChemica PanReac AppliChem A1492
Penicillin G Potassium Salt BioChemica PanReac AppliChem A1837
Lipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880
Critical Commercial Assays
Human IL-1β uncoated ELISA Invitrogen 88-7261
Human TNF-α uncoated ELISA Invitrogen 88-7346
Human IL-6 ELISA Invitrogen 88-7066
Human IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176
Human Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE
Human IFN-γ ELISA Diaclone 950.000.192
Software and Algorithms
GraphPad Prism Graphpad Software https://www.graphpad.com
SPSS IBM https://www.ibm.com/analytics/spss-statistics-software