Target Pathogen Working Electrode Biorecognition Element Electrochemical Method & Probe Limit of Detection Secondary Binding Step Reference E. coli ITO electrode anti-E. coli EIS; Fe(CN)63-/4- 6 × 105 cells/mL antibody/ALP conjugate label for amplification Yang and Li (2005) V. cholerae carbon/polystyrene electrode polyclonal anti-V.cholerae chrono-amperometry 105 cells/mL antibody-ALP conjugate label for amplification Rao et al. (2006) E. coli Au interdigitated microelectrode array polyclonal anti-E. coli EIS 2.67 × 106 cells/mL antibody-coated MBs for separation Varshney et al. (2007) V. parahaemolytic carbon electrode anti-V. parahaemolytic CV; thionine/hydrogen peroxide 7.37 × 104 CFU/mL antibody/HRP conjugate label for transduction Zhao et al. (2007) E. coli Au interdigitated microelectrode array polyclonal anti-E. coli EIS 7.4 × 104 CFU/mL antibody-coated MBs for separation and amplification Varshney and Li (2007) E. coli AuNPs on carbon electrode monoclonal and polyclonal anti-E. coli CV; ferrocenedicarboxylic acid/hydrogen peroxide 6 CFU/mL polyclonal antibody/HRP conjugate label for amplification Lin et al. (2008) S. aureus Au electrode anti-S. aureus amperometry; tetrathiafulvalene/hydrogen peroxide 370 cells/mL antibody/HRP conjugate label for amplification Escamilla-Gomez et al. (2008) S. typhimurium Au electrode monoclonal anti-S. typhimurium chrono-amperometry; tetramethylbenzidine dihydrochloride/hydrogen peroxide 21 CFU/mL anti-S. typhimurium polyclonal antibody/HRP conjugate label for amplification Salam and Tothill (2009) S. typhimurium graphite-epoxy composite electrode polyclonal anti-S. typhimurium amperometry 0.1 CFU/mL primary antibody-coated MBs for separation, secondary antibody/HRP conjugate label for amplification Liebana et al. (2009) avian influenza virus (AIV) H5N1 Au interdigitated microelectrode array monoclonal anti-AIV-H5 EIS 0.26 HAU/mL antibody-coated MBs for separation Wang et al. (2010) Streptococcus pneumoniae Au electrode polyclonal anti-S. pneumoniae amperometry; tetrathiafulvalene/hydrogen peroxide 1.5 × 104 CFU/mL antibody-coated MBs for separation and bacteria immobilization, antibody/HRP conjugate label for amplification Campuzano et al. (2010) E. coli carbon-graphite electrode monoclonal anti-E. coli CV 7 CFU/mL antibody-coated MBs for separation, antibody/polyaniline label for amplification Setterington and Alocilja (2011) S. aureus MBs on Au electrode polyclonal anti-Protein A (S. aureus) amperometry; tetrathiafulvalene/hydrogen peroxide 1 CFU/mL antibody/Protein A/HRP conjugate for amplification Esteban-Fernandez de Avila et al. (2012) avian influenza virus (AIV) H5N1 Au interdigitated microelectrode array monoclonal anti-AIV-H5, polyclonal anti-AIV-N1 EIS 103 EDI50/mL anti-AIV-H5 monoclonal antibody- coated MBs for separation, red blood cell label for amplification Lum et al. (2012) E. coli AuNPs/SiO2 nanocomposite on sulfhydryl chitosan/Fe(C2H5)2/C60 composite on carbon electrode monoclonal anti-E. coli CV; ferrocene 15 CFU/mL antibody/glucose oxidase/Pt nanochain conjugate label for amplification Li et al. (2013) C. parvum polypyrrole-coated carbon electrode polyclonal anti-C. parvum chrono-potentiometry; o-phenylenediamine/hydrogen peroxide 500 oocysts/mL antibody/HRP conjugate label for amplification Laczka et al. (2013) L. monocytogenes polymeric ion-selective membrane electrode anti-L. monocytogenes InlA aptamer potentiometry 10 CFU/mL aptamer/protamine label for transduction Ding et al. (2014) avian influenza virus (AIV) H5N1 Au interdigitated electrode array anti-AIVH5N1 aptamer EIS 0.04 HAU/mL aptamer-coated MBs for separation, Concanavalin A/glucose oxide-coated AuNP labels for amplification Fu et al. (2014). L. monocytogenes interdigitated microelectrode array monoclonal and polyclonal anti-L. monocytogenes EIS 300 CFU/mL monoclonal antibody-coated MBs for separation, polyclonal antibody-coated AuNP label for secondary binding amplification Chen et al. (2015) E. coli carbon electrode polyclonal anti-E.coli chrono-amperometry 148 CFU/mL primary antibody-coated MBs for separation, secondary antibody-coated AuNPs for amplification Hassan et al. (2015) avian influenza virus (AIV) H5N1 AuNPs on ITO microelectrode polyclonal anti-AIVH5N1 ASV 10 pg/mL antibody-coated MBs for separation and anodic stripping Zhou et al. (2015) E. coli Au interdigitated microelectrode array anti-E.coli EIS; Fe(CN)63-/4- 100 CFU/mL wheat germ agglutinin for amplification Li et al. (2015) E. coli carbon electrode monoclonal and polyclonal anti-E. coli DPV 10 CFU/mL monoclonal antibody-coated MBs for separation, polyclonal antibody-coated AuNP label for amplification Wang and Alocilja (2015) norovirus nanostructured Au microelectrode concanavalin A lectin, polyclonal anti-norovirus CV, EIS; Fe(CN)63-/4- 35 copies/mL antibody-ALP conjugate label for amplification Hong et al. (2015) Legionella pneumophila carbon electrode polyclonal anti-L. pneumophila amperometry; hydroquinone/hydrogen peroxide 10 CFU/mL primary antibody- coated MBs for separation, secondary antibody/HRP conjugate label for amplification Martin et al. (2015) S. aureus carbon electrode anti-S.aureus aptamer ASV 1 CFU/mL primary aptamer-coated MBs for separation, secondary aptamer-coated AgNP label for anodic stripping Abbaspour et al. (2015) L. monocytogenes Au interdigitated microelectrode array monoclonal and polyclonal anti-L. monocytogenes EIS 160 CFU/mL monoclonal antibody-coated MBs for separation, polyclonal antibody-coated AuNP label for amplification Chen et al. (2016b) E. coli Au interdigitated microelectrode array polyclonal anti-E. coli CV, amperometry 52 CFU/mL antibody-coated, AuNP/glucose oxidase-modified MBs for separation and amplification Xu et al. (2016a) E. coli Au interdigitated microelectrode array anti- E. coli EIS 100 CFU/mL antibody-coated MBs for separation, antibody/glucose oxidase conjugate for amplification Xu et al. (2016b) S. typhimurium Au interdigitated microelectrode array monoclonal anti-S. typhimurium EIS 100 CFU/mL antibody-coated MBs for separation, antibody/glucose oxidase conjugate label for amplification Xu et al. (2016b) E. coli chitosan/CNT composite on carbon electrode polyclonal anti-E. coli CV; thionine/hydrogen peroxide 50 CFU/mL secondary antibody/HRP conjugate label enzyme-assisted reduction reaction Gayathri et al. (2016) S. typhimurium carbon electrode polyclonal and monoclonal anti-S. typhimurium DPV 100 cells/mL polyclonal antibody- coated MBs for separation, monoclonal antibody- coated AuNP label for amplification Afonso et al. (2016) E. coli Au electrode anti-E. coli EIS; Fe(CN)63-/4- 100 CFU/mL AuNP label for amplification Wan et al. (2016) L. monocytogenes Au interdigitated electrode array polyclonal anti-L. monocytogenes EIS 1.6 × 103 CFU/mL antibody-coated MBs for separation, antibody-coated AuNP label for amplification Wang et al. (2017) E. coli Au microelectrode monoclonal anti-E. coli LSV 39 CFU/mL antibody-coated MBs for separation, antibody/AuNP/nucleotide/CdSNP conjugate label for amplification Li et al. (2017) V. cholerae Au microelectrode polyclonal anti-V. cholerae LSV 32 CFU/mL antibody-coated MBs for separation, antibody/AuNP/nucleotide/PbSNP conjugate label for amplification Li et al. (2017) avian influenza virus (AIV) H5N1 Au electrode anti-AIVH5N1, concanavalin A lectin CV 0.367 HAU/mL Concanavalin A- coated MB labels for amplification Zhang et al. (2017) human influenza A virus H9N2 carbon electrode polyclonal anti-influenza A virus M2 protein, fetuin A chrono-amperometry 16 HAU antibody-coated MBs for separation, fetuin A-coated AuNP label for amplification Sayhi et al. (2018) human enterovirus 71 (EV71) AuNPs on ITO electrode monoclonal anti-EV71 CV, EIS, colorimetry; Fe(CN)63-/4- 10 pg/mL antibody/HRP-coated MB labels for amplification Hou et al. (2018) E. coli Ag interdigitated microelectrode array melittin peptide EIS 1 CFU/mL MLT-coated MBs used for separation and bacteria immobilization Wilson et al. (2019) S. typhimurium Ag interdigitated electrode array melittin peptide EIS 10 CFU/mL MLT-coated MBs used for separation and bacteria immobilization Wilson et al. (2019) S. aureus Ag interdigitated electrode array melittin peptide EIS 110 CFU/mL MLT-coated MBs used for separation and bacteria immobilization Wilson et al. (2019) Middle East respiratory syndrome corona virus (MERS-CoV) AuNPs on carbon electrode MERS-CoV antigen-antibody complex SWV; Fe(CN)63-/4- 400 fg/mL MERS CoV-antibody complex Layqah and Eissa (2019)