Redox reactions at the electrode-electrolyte interface are typically established using a redox probe. Owing to its high reversibility, the Fe(CN)6 3 - /4- redox couple has been widely investigated as an electrochemical probe for biosensing applications and is regarded as a standard model for highly reversible electrochemical reactions (Daum and Enke, 1969). While useful electrochemical probes, redox reactions may also affect the electrode and immobilized biorecognition elements. For example, redox reactions associated with the Fe(CN)6 3 - /4- probe can cause etching of Au electrodes due to the presence of CN− ions when using the redox couple for EIS measurements (Vogt et al. 2016). This observation warrants further investigation, particularly in the context of establishing the effects on biosensor repeatability and reusability. The use of alternative redox probes or electrode materials may mitigate such effects. For example, ferrocene and ferrocenemethanol have also been used as redox probes for pathogen detection. Ruthenium(III)/ruthenium(II) (Schrattenecker et al. 2019) and immobilized quinone pairs (Piro et al. 2013) are also potentially useful alternatives.