Coltiviruses (genus: Coltivirus, family: Reoviridae) are double-strand segmented RNA viruses. Currently, only two viruses have been defined in the genus: Colorado tick fever virus (CTFV) found in North America, and Eyach virus (EYAV) from Europe. Their RNA genome comprises twelve segments. Both viruses can cause severe disease in humans (Attoui et al. 2005; Moutailler et al. 2016), and are transmitted by ticks (Attoui et al. 2002). Recently, several novel coltiviruses have been characterized, including Tarumizu tick virus from Haemaphysalis flava ticks in Japan (Fujita et al. 2017), Kundal virus from Hyalomma anatolicum ticks in India (Yadav et al. 2019), Shelly headland virus (SHLV) from Ixodes holocyclus ticks in Australia (Harvey et al. 2018), and Tai Forest reovirus (TFRV) from free-tailed bats (Chaereophon aloysiisabaudiae) in Côte d’Ivoire (Weiss et al. 2017). Hence, there is evidently a high diversity of coltiviruses in nature and many more are likely to be identified. To date, however, it is unknown whether coltiviruses are associated with wildlife disease.