3.6 Molecular investigation of DYPV and LSPV in local ticks
As DYPV was identified in ticks (A. javanense) collected from pangolin 1-Dongyang, and LSPV was closely related to SHLV also identified in ticks (I. holocyclus) from Australia, we collected ticks at the locations from where the sick pangolins were found. Consequently, 452 ticks representing 7 species were collected, including 220 Haemaphysalis hystricis, 147 Rhipicephalus microplus, 49 Haemaphysalis longicornis, 11 Ixodes granulatus, 11 Rhipicephalus haemaphysaloides, 9 Rhipicephalus sanguineus, and 5 Haemaphysalis mageshimaensis. Unfortunately, neither DYPV nor LSPV were identified in these ticks by meta-transcriptomics and nested RT-PCR.