Authors’ contributions: PS: conceived the study, coordinated the in vitro virus cultivation and wrote the manuscript together with FL and ALP; SFiore, EB,AM,CF prepared the Vero cells and cultivated the two virus; GF, AF, RDS and SFillo performed the whole genome sequence of the new genomes (ID: EPI_ISL_412973 and 412974); ALP, AA, AC analysed the genomes together with GF and FL; MRCapobianchi and MRG diagnosed SARS-CoV-2 onto the two samples; AC set up the molecular methods; MRCastrucci coordinated laboratory confirmation; GR read and revised the manuscript. The authors read and approved the manuscript.