PMC:7120874 / 18456-19490 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7120874","sourcedb":"PMC","sourceid":"7120874","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7120874","text":"CM10 Chips\nAdd 90 µL of 100 mM sodium acetate buffer at pH 4.0 (CM10 chip binding buffer) into each well in the bioprocessor set up containing the CM10 chips.\nAdd 10 µL of each ion exchange bead eluate (sample fractions 1–6) to the well for sample binding.\nMix well by shaking for 30 min at RT.\nRemove the sample fractions.\nWash the chip spot in the well by adding 150 µL of CM10 chip binding buffer again into each well and shake for 5 min at RT.\nRemove buffer.\nRepeat washing (steps 5 and 6) once more.\nRinse with water two times by adding 200 µL of Milli-Q water to each well and discard immediately.\nRemove the chips from bioprocessor and air-dry the chips for 5 min.\nAdd 1 µL of 50% sinapinic acid to each chip spot.\nAir-dry the chip for around 10 min.\nAdd 1 µL of 50% sinapinic acid and air-dry again.\nThe chip is ready to be read in the PBS IIc mass spectrometer reader.\nProcess at least one reference control serum concurrently with the patients’ samples on each chip for quality control of chip-to-chip variability as before.","divisions":[{"label":"title","span":{"begin":0,"end":10}},{"label":"p","span":{"begin":11,"end":158}},{"label":"p","span":{"begin":159,"end":256}},{"label":"p","span":{"begin":257,"end":294}},{"label":"p","span":{"begin":295,"end":323}},{"label":"p","span":{"begin":324,"end":447}},{"label":"p","span":{"begin":448,"end":462}},{"label":"p","span":{"begin":463,"end":504}},{"label":"p","span":{"begin":505,"end":603}},{"label":"p","span":{"begin":604,"end":671}},{"label":"p","span":{"begin":672,"end":721}},{"label":"p","span":{"begin":722,"end":757}},{"label":"p","span":{"begin":758,"end":807}},{"label":"p","span":{"begin":808,"end":877}}],"tracks":[]}