8 mL Blood is collected from each patient or control subject.
Incubate the blood for approx 2 h at 4°C to clot the blood cells.
Separate the serum portion from the clotted cells by centrifugation at 230g for 15 min.
Aliquot the sera for various routine laboratory diagnostic tests.
Freeze the remaining sera at −70°C for protein chip array profiling analysis.