0 were loaded and the plates were incubated at 37 °C for 60 min. IBV-specific IgG was detected with anti-chicken IgY (IgG) (whole molecule)-peroxidase antibodies produced in rabbits (Sigma-Aldrich, Germany). After incubated with 3,3′,5,5′-Tetramethylbenzidine(TMB) substrate for 15 min a