Identification of 28 exact matches to SARS CoV immunogenic peptides by screening all epitopes deposited in IEDB We collected all peptides in IEDB ( 3, as of 13-02-2020) reported positive in T cell assays and have human as the host organism. We then conducted a local sequence alignment of 10 2019-nCoV open reading frames (ORFs) against 35,225 IEDB peptides, and found 28 exact matches. Surprisingly, all identical hits (against peptides having sequence length greater than 3) were from SARS-CoV ( Table 1, Data Table 1 4). These peptides have been shown to bind various HLA alleles, although with higher tendency towards HLA-A:02:01, from both class I and class II, and can be target for CD8+ and CD4+ T cells respectively. Table 1. 28 2019-nCoV peptides having exact matches with immunogenic SARS-CoV peptides. IEDB.peptide 2019-nCoV.pattern Antigen.Name Allele.Name TLACFVLAAV TLACFVLAAV Membrane glycoprotein HLA-A *02:01 AFFGMSRIGMEVTPSGTW AFFGMSRIGMEVTPSGTW N protein ALNTPKDHI ALNTPKDHI Nucleoprotein HLA-A *02:01 AQFAPSASAFFGMSR AQFAPSASAFFGMSR nucleocapsid protein HLA class II AQFAPSASAFFGMSRIGM AQFAPSASAFFGMSRIGM N protein GMSRIGMEV GMSRIGMEV Nucleoprotein HLA-A *02:01 ILLNKHIDA ILLNKHIDA Nucleoprotein HLA-A *02:01 IRQGTDYKHWPQIAQFA IRQGTDYKHWPQIAQFA N protein KHWPQIAQFAPSASAFF KHWPQIAQFAPSASAFF N protein LALLLLDRL LALLLLDRL Nucleoprotein HLA-A *02:01 LLLDRLNQL LLLDRLNQL Nucleoprotein HLA-A *02:01 LLNKHIDAYKTFPPTEPK LLNKHIDAYKTFPPTEPK N protein LQLPQGTTL LQLPQGTTL Nucleoprotein HLA-A *02:01 RRPQGLPNNTASWFT RRPQGLPNNTASWFT nucleocapsid protein HLA class I YKTFPPTEPKKDKKKK YKTFPPTEPKKDKKKK N protein ILLNKHID ILLNKHID Nucleoprotein HLA-A *02:01 MEVTPSGTWL MEVTPSGTWL nucleocapsid protein HLA-B *40:01 ALNTLVKQL ALNTLVKQL S protein HLA-A *02:01 FIAGLIAIV FIAGLIAIV Spike glycoprotein precursor HLA-A2 LITGRLQSL LITGRLQSL Spike glycoprotein precursor HLA-A2 NLNESLIDL NLNESLIDL S protein HLA-A *02:01 QALNTLVKQLSSNFGAI QALNTLVKQLSSNFGAI S protein HLA-DRB1 *04:01 RLNEVAKNL RLNEVAKNL Spike glycoprotein precursor HLA-A *02:01 VLNDILSRL VLNDILSRL S protein HLA-A *02:01 VVFLHVTYV VVFLHVTYV Spike glycoprotein precursor HLA-A *02:01 GAALQIPFAMQMAYRF GAALQIPFAMQMAYRF S protein HLA-DRA *01:01/DRB1 *07:01 MAYRFNGIGVTQNVLY MAYRFNGIGVTQNVLY S protein HLA-DRB1 *04:01 QLIRAAEIRASANLAATK QLIRAAEIRASANLAATK S protein HLA-DRB1 *04:01 *SARS-CoV: Severe acute respiratory syndrome coronavirus