Diagnosis of coronaviruses is unnecessary in most cases of self-limited infection, as most of the patients have mild or moderate syndromes with good prognosis. However, it may be essential to identify an etiological agent in epidemiological studies, especially during the epidemics outbreak. Since 2019-nCoV has not been found before in humans, there is no vaccine or special treatment for it so far. The number of cases is increasing rapidly, even under the current public health emergency. Thus, diagnosing all suspected cases as soon as possible and immediately isolating them is critical to cutting off the source of infection. Conventional diagnostic testing methods, such as the assays for detection of antiviral antibodies or viral antigens have been developed and used clinically. New diagnostic strategies including multiplex nucleic acid amplification and microarray-based assays may help in epidemiologic monitoring, along with taking effective prevention steps [37,38]. 2019-nCoV nucleic acids can be detected in specimens including nasopharyngeal swabs, sputum, lower respiratory tract secretions, blood and stool [20].