PMC:7068163 / 6962-7693 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7068163","sourcedb":"PMC","sourceid":"7068163","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7068163","text":"Figure 2 Example image of real-time RT-PCR curves of the E gene assay with unspecific signals at late cycles, Bavaria, February 2020\nRFU: relative fluorescence units.\nCurves: 1: Wuhan coronavirus 2019 E gene positive control; 2: SARS-CoV Frankfurt 1 RNA positive control; 3,4,6,8: negative patient samples; 5: extraction negative control; 7: non-template negative control.\nSignal is given in log scale with threshold = 200. PCR was performed with SuperScript III system and E gene primers and probe as published in [5]. Curves of positive controls (1 and 2) show expected sigmoid curves. Curves 3–6 show unspecific signals with increase above threshold. Curves below threshold were not considered as significant signals (7 and 8).","divisions":[{"label":"label","span":{"begin":0,"end":8}},{"label":"caption","span":{"begin":10,"end":133}},{"label":"p","span":{"begin":10,"end":133}},{"label":"p","span":{"begin":134,"end":167}},{"label":"p","span":{"begin":168,"end":373}}],"tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"111","span":{"begin":58,"end":59},"obj":"Gene"},{"id":"113","span":{"begin":134,"end":137},"obj":"Chemical"},{"id":"118","span":{"begin":202,"end":203},"obj":"Gene"},{"id":"119","span":{"begin":179,"end":196},"obj":"Species"},{"id":"120","span":{"begin":230,"end":238},"obj":"Species"},{"id":"121","span":{"begin":291,"end":298},"obj":"Species"},{"id":"123","span":{"begin":475,"end":476},"obj":"Gene"}],"attributes":[{"id":"A111","pred":"tao:has_database_id","subj":"111","obj":"Gene:43740570"},{"id":"A118","pred":"tao:has_database_id","subj":"118","obj":"Gene:43740570"},{"id":"A119","pred":"tao:has_database_id","subj":"119","obj":"Tax:2697049"},{"id":"A120","pred":"tao:has_database_id","subj":"120","obj":"Tax:694009"},{"id":"A121","pred":"tao:has_database_id","subj":"121","obj":"Tax:9606"},{"id":"A123","pred":"tao:has_database_id","subj":"123","obj":"Gene:43740570"},{"subj":"111","pred":"source","obj":"LitCovid-PubTator"},{"subj":"113","pred":"source","obj":"LitCovid-PubTator"},{"subj":"118","pred":"source","obj":"LitCovid-PubTator"},{"subj":"119","pred":"source","obj":"LitCovid-PubTator"},{"subj":"120","pred":"source","obj":"LitCovid-PubTator"},{"subj":"121","pred":"source","obj":"LitCovid-PubTator"},{"subj":"123","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T25","span":{"begin":60,"end":64},"obj":"Body_part"},{"id":"T26","span":{"begin":204,"end":208},"obj":"Body_part"},{"id":"T27","span":{"begin":251,"end":254},"obj":"Body_part"},{"id":"T28","span":{"begin":477,"end":481},"obj":"Body_part"}],"attributes":[{"id":"A25","pred":"fma_id","subj":"T25","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A26","pred":"fma_id","subj":"T26","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A27","pred":"fma_id","subj":"T27","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A28","pred":"fma_id","subj":"T28","obj":"http://purl.org/sig/ont/fma/fma74402"},{"subj":"T25","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T26","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T27","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T28","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T3","span":{"begin":397,"end":402},"obj":"Body_part"}],"attributes":[{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T32","span":{"begin":230,"end":238},"obj":"Disease"}],"attributes":[{"id":"A32","pred":"mondo_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"subj":"T32","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T69","span":{"begin":60,"end":64},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T70","span":{"begin":87,"end":94},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T71","span":{"begin":204,"end":208},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T72","span":{"begin":374,"end":380},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T73","span":{"begin":477,"end":481},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T74","span":{"begin":596,"end":599},"obj":"http://purl.obolibrary.org/obo/CLO_0001313"},{"id":"T75","span":{"begin":616,"end":623},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T76","span":{"begin":713,"end":720},"obj":"http://purl.obolibrary.org/obo/SO_0000418"}],"attributes":[{"subj":"T69","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T70","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T71","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T72","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T73","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T74","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T75","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T76","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T14","span":{"begin":494,"end":499},"obj":"Chemical"}],"attributes":[{"id":"A14","pred":"chebi_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"subj":"T14","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T53","span":{"begin":0,"end":133},"obj":"Sentence"},{"id":"T54","span":{"begin":134,"end":167},"obj":"Sentence"},{"id":"T55","span":{"begin":168,"end":175},"obj":"Sentence"},{"id":"T56","span":{"begin":176,"end":178},"obj":"Sentence"},{"id":"T57","span":{"begin":179,"end":373},"obj":"Sentence"},{"id":"T58","span":{"begin":374,"end":424},"obj":"Sentence"},{"id":"T59","span":{"begin":425,"end":520},"obj":"Sentence"},{"id":"T60","span":{"begin":521,"end":588},"obj":"Sentence"},{"id":"T61","span":{"begin":589,"end":654},"obj":"Sentence"},{"id":"T62","span":{"begin":655,"end":731},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T53","pred":"source","obj":"LitCovid-sentences"},{"subj":"T54","pred":"source","obj":"LitCovid-sentences"},{"subj":"T55","pred":"source","obj":"LitCovid-sentences"},{"subj":"T56","pred":"source","obj":"LitCovid-sentences"},{"subj":"T57","pred":"source","obj":"LitCovid-sentences"},{"subj":"T58","pred":"source","obj":"LitCovid-sentences"},{"subj":"T59","pred":"source","obj":"LitCovid-sentences"},{"subj":"T60","pred":"source","obj":"LitCovid-sentences"},{"subj":"T61","pred":"source","obj":"LitCovid-sentences"},{"subj":"T62","pred":"source","obj":"LitCovid-sentences"}]},{"project":"2_test","denotations":[{"id":"32156330-31992387-29325821","span":{"begin":517,"end":518},"obj":"31992387"}],"attributes":[{"subj":"32156330-31992387-29325821","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"32156330-31992387-29325821","span":{"begin":517,"end":518},"obj":"31992387"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"32156330-31992387-29325821","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#ec93d5","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#93ece9"},{"id":"LitCovid-PD-UBERON","color":"#eccf93"},{"id":"LitCovid-PD-MONDO","color":"#b593ec"},{"id":"LitCovid-PD-CLO","color":"#93ec9b"},{"id":"LitCovid-PD-CHEBI","color":"#ec93a5"},{"id":"LitCovid-sentences","color":"#93bfec"},{"id":"2_test","color":"#d9ec93"},{"id":"MyTest","color":"#e593ec"}]}]}}