Table 1  SARS-CoV-2 PCR cycling conditions and software settings in the Utility Channel software used to create the run template
Sample type  Alcohol-based swab (400 µL input)
Channels  1  2:SARS-CoV-2 E-gene  3  4  IC
RFI  NA  1.5  NA  NA  Predefined
PCR cycling conditions  UNG incubation  Pre-PCR step  1st measurement  2nd measurement  Cooling
Number of cycles  Predefined  1  5  45  Predefined
Number of steps  3  2  2
Temperature  55 °C; 60 °C; 65 °C  95 °C; 55 °C  91 °C; 58 °C
Hold time  120 s; 360 s; 240 s  5 s; 30 s  5 s; 25 s
Data acquisition  None  End of each cycle  End of each cycle
IC: internal control; NA: not applicable; RFI: relative fluorescence increase; UNG: uracil-DNA N-glycosylase.