PMC:7036342 / 4008-4607 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"102","span":{"begin":257,"end":271},"obj":"Chemical"},{"id":"103","span":{"begin":279,"end":291},"obj":"Chemical"}],"attributes":[{"id":"A102","pred":"tao:has_database_id","subj":"102","obj":"MESH:C005460"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"In order to observe virus particles, Vero cell monolayer showing the cytopathic effects was fixed as previously described.7 It was cut on ultramicrotome (RMC MT-XL; RMC Boeckeler, Tucson, AZ, USA) at 65 nm. Ultrathin sections were stained with saturated 4% uranyl acetate and 1% lead citrate before examination with a transmission electron microscope (JEM-1400; JEOL USA Inc., Peabody, MA, USA) at 80 kV. Spherical particles with crown-like spikes ranging 66 to 81 nm in diameter were observed within the cytoplasmic vesicles and in the extracellular space adjacent to cell membrane (Fig. 1C and D)."}

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T117","span":{"begin":20,"end":25},"obj":"NCBITaxon:10239"},{"id":"T116","span":{"begin":37,"end":41},"obj":"CL_6"},{"id":"T115","span":{"begin":331,"end":339},"obj":"CHEBI:10545;CHEBI:10545"},{"id":"T114","span":{"begin":505,"end":525},"obj":"GO:0031410"},{"id":"T113","span":{"begin":537,"end":556},"obj":"GO:0005615"},{"id":"T112","span":{"begin":569,"end":582},"obj":"GO:0005886"}],"text":"In order to observe virus particles, Vero cell monolayer showing the cytopathic effects was fixed as previously described.7 It was cut on ultramicrotome (RMC MT-XL; RMC Boeckeler, Tucson, AZ, USA) at 65 nm. Ultrathin sections were stained with saturated 4% uranyl acetate and 1% lead citrate before examination with a transmission electron microscope (JEM-1400; JEOL USA Inc., Peabody, MA, USA) at 80 kV. Spherical particles with crown-like spikes ranging 66 to 81 nm in diameter were observed within the cytoplasmic vesicles and in the extracellular space adjacent to cell membrane (Fig. 1C and D)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T21","span":{"begin":42,"end":46},"obj":"Body_part"},{"id":"T22","span":{"begin":505,"end":525},"obj":"Body_part"},{"id":"T23","span":{"begin":537,"end":556},"obj":"Body_part"},{"id":"T24","span":{"begin":569,"end":582},"obj":"Body_part"},{"id":"T25","span":{"begin":569,"end":573},"obj":"Body_part"}],"attributes":[{"id":"A21","pred":"fma_id","subj":"T21","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A22","pred":"fma_id","subj":"T22","obj":"http://purl.org/sig/ont/fma/fma63175"},{"id":"A23","pred":"fma_id","subj":"T23","obj":"http://purl.org/sig/ont/fma/fma70022"},{"id":"A24","pred":"fma_id","subj":"T24","obj":"http://purl.org/sig/ont/fma/fma63841"},{"id":"A25","pred":"fma_id","subj":"T25","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"In order to observe virus particles, Vero cell monolayer showing the cytopathic effects was fixed as previously described.7 It was cut on ultramicrotome (RMC MT-XL; RMC Boeckeler, Tucson, AZ, USA) at 65 nm. Ultrathin sections were stained with saturated 4% uranyl acetate and 1% lead citrate before examination with a transmission electron microscope (JEM-1400; JEOL USA Inc., Peabody, MA, USA) at 80 kV. Spherical particles with crown-like spikes ranging 66 to 81 nm in diameter were observed within the cytoplasmic vesicles and in the extracellular space adjacent to cell membrane (Fig. 1C and D)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T44","span":{"begin":20,"end":25},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T45","span":{"begin":37,"end":46},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T46","span":{"begin":154,"end":157},"obj":"http://purl.obolibrary.org/obo/CLO_0008845"},{"id":"T47","span":{"begin":165,"end":168},"obj":"http://purl.obolibrary.org/obo/CLO_0008845"},{"id":"T48","span":{"begin":316,"end":317},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T49","span":{"begin":569,"end":573},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T50","span":{"begin":574,"end":582},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"}],"text":"In order to observe virus particles, Vero cell monolayer showing the cytopathic effects was fixed as previously described.7 It was cut on ultramicrotome (RMC MT-XL; RMC Boeckeler, Tucson, AZ, USA) at 65 nm. Ultrathin sections were stained with saturated 4% uranyl acetate and 1% lead citrate before examination with a transmission electron microscope (JEM-1400; JEOL USA Inc., Peabody, MA, USA) at 80 kV. Spherical particles with crown-like spikes ranging 66 to 81 nm in diameter were observed within the cytoplasmic vesicles and in the extracellular space adjacent to cell membrane (Fig. 1C and D)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T17","span":{"begin":158,"end":160},"obj":"Chemical"},{"id":"T18","span":{"begin":264,"end":271},"obj":"Chemical"},{"id":"T20","span":{"begin":284,"end":291},"obj":"Chemical"},{"id":"T23","span":{"begin":331,"end":339},"obj":"Chemical"},{"id":"T24","span":{"begin":386,"end":388},"obj":"Chemical"},{"id":"T27","span":{"begin":430,"end":435},"obj":"Chemical"}],"attributes":[{"id":"A17","pred":"chebi_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/CHEBI_73614"},{"id":"A18","pred":"chebi_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/CHEBI_30089"},{"id":"A19","pred":"chebi_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/CHEBI_47622"},{"id":"A21","pred":"chebi_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/CHEBI_16947"},{"id":"A22","pred":"chebi_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/CHEBI_50744"},{"id":"A20","pred":"chebi_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/CHEBI_133748"},{"id":"A23","pred":"chebi_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/CHEBI_10545"},{"id":"A24","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_474859"},{"id":"A25","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_73610"},{"id":"A26","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_90325"},{"id":"A27","pred":"chebi_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/CHEBI_37409"}],"text":"In order to observe virus particles, Vero cell monolayer showing the cytopathic effects was fixed as previously described.7 It was cut on ultramicrotome (RMC MT-XL; RMC Boeckeler, Tucson, AZ, USA) at 65 nm. Ultrathin sections were stained with saturated 4% uranyl acetate and 1% lead citrate before examination with a transmission electron microscope (JEM-1400; JEOL USA Inc., Peabody, MA, USA) at 80 kV. Spherical particles with crown-like spikes ranging 66 to 81 nm in diameter were observed within the cytoplasmic vesicles and in the extracellular space adjacent to cell membrane (Fig. 1C and D)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T22","span":{"begin":0,"end":206},"obj":"Sentence"},{"id":"T23","span":{"begin":207,"end":404},"obj":"Sentence"},{"id":"T24","span":{"begin":405,"end":599},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In order to observe virus particles, Vero cell monolayer showing the cytopathic effects was fixed as previously described.7 It was cut on ultramicrotome (RMC MT-XL; RMC Boeckeler, Tucson, AZ, USA) at 65 nm. Ultrathin sections were stained with saturated 4% uranyl acetate and 1% lead citrate before examination with a transmission electron microscope (JEM-1400; JEOL USA Inc., Peabody, MA, USA) at 80 kV. Spherical particles with crown-like spikes ranging 66 to 81 nm in diameter were observed within the cytoplasmic vesicles and in the extracellular space adjacent to cell membrane (Fig. 1C and D)."}