Figure 1  P. aeruginosa is able to suppress antiviral response of airway epithelial cells. (A) Cell culture based experimental workflow. (B) BEAS2B cells were pretreated with conditioned medium of P. aeruginosa (PAO1 and Boston) or control medium and subsequently infected with RSV or RV1b. Induction of MX1 and OAS1 mRNA were analyzed by qRT-PCR after 6, 10, and 14 h. (C) BEAS2B cells were treated as in (A) and cell culture supernatant of RV1b (human RV) or RSV infected cells (14 h) was transferred to new BEAS2B cells. Levels of RV1b (hRV) or RSV mRNA were analyzed by qRT-PCR and the number of wells displaying RV1b or RSV specific cytopathogenic effects (CPE) was determined. (D) Primary HBE cells were pretreated with conditioned medium of P. aeruginosa (PAO1) or control medium and subsequently infected with RSV. Induction of MX1 and OAS1 mRNA were analyzed by qRT-PCR after 14 h. (E) BEAS2B cells were pretreated with conditioned medium of P. aeruginosa or control medium and subsequently infected with RSV. Induction of MX1 and OAS1 mRNA were analyzed by qRT-PCR after 14 h. All experiments n = 3–4, ANOVA and Bonferroni post-test was used for statistical analysis. Significant differences were considered at *p < 0.05, **p < 0.01, and ***p < 0.001 as compared to the control condition. n.s., not significant.