CF patients with intermittent P. aeruginosa infection are at higher risk to be infected with hRV. (A) Capacity of CF P. aeruginosa isolates to degrade IFNλ was analyzed by western blot and protease activity was measured by skim milk agar. Correlation was assessed using Spearman analysis (n = 51). (B) P. aeruginosa isolates from (A) were classified according to infection status and plotted against relative IFNλ degradation after 1 h. Statistical analysis was done using non-parametric Mann-Whitney test. (C) Presence of respiratory viruses in sputum samples of CF patients were assessed using a multiplex diagnostic virus panel assay. Relative occurrence of respiratory virus positive samples and total samples are indicated (n = 340). (D) The prevalence of hRV in CF samples (nose, throat and sputum) was plotted according to the infection status (n = 340). Statistics was done by using Fisher's Exact Test. (E) The prevalence of hRV in CF sputum samples was plotted according to the infection status (n = 499). Statistics was done by using Fisher's Exact Test. (F) Presence of hRV in sputum from CF patients was assessed by qPCR. The Ct values of hRV was plotted according to the infection status (n = 24). Significant differences were considered at *p < 0.05, **p < 0.01, and ***p < 0.001 as compared to the control condition. n.s., not significant.