Transfection
Transfection of primary CD4+ T-cells was carried out using “Nucleofector Kit for T-cells” according to the manufacturer's protocol (Lonza Group, Basel Switzerland). miR-146a-5p mimic and mimic “Negative control #1” (Dharmacon, Lafayette CO) were used for transfection at a concentration of 20 nM. miR-146a-5p stem-loop miRNA inhibitor and stem-loop miRNA inhibitor “Negative control #1” (Dharmacon, Lafayette CO) were used for transfection at a concentration of 40 nM. Transfected cells were harvested at 48 h post transfection.