Meth enhances IL-1β expression and Caspase-1 activation in CD4+ T-cells. CD4+ T-cells were treated daily with or without Meth. (A) Expression of IL-1β was determined from cell culture supernatants by ELISA analysis. Relative expression was calculated by normalizing Meth treated samples to untreated control cells. Data represent the mean ± SD of 3 independent experiments, and p-values were calculated relative to untreated controls (*p < 0.05, **p < 0.01). (B) CD4+ T-cells were untreated, treated with Meth, or treated with Nigericin. Caspase-1 Activation was measured using fluorescent labeling with FAM-FLICA, and analyzed by Flow Cytometry. Data represent the mean ± SD of 3 independent experiments, and p-values were calculated relative to untreated controls (***p < 0.001).