Table 1 Nucleotide and amino acid differences of spike gene between the virulent iPEDVPT-P5 and attenuated iPEDVPT-P96 Viruses. Position in Spike Gene Nucleotide Amino Acid Nucleotide Amino Acid Domain iPEDVPT-P5 iPEDVPT-P96 iPEDVPT-P5 iPEDVPT-P96 431 144 S10 C T T I 1661 554 S1B T C F S 2659 887 S2 A C S R 2902 968 S2 T G S A 3062 1021 S2 HR1 † T G I S 3077 1026 S2 HR1 † A G K R 3755 1252 S2 T G L R 4061 1354 S2 CT ‡ G T C F 4073 1358 S2 CT ‡ G T C F 4134 1378 S2 CT ‡ A T E D † HR1: heptad repeat 1; ‡ CT: cytoplasmic tail.