In vivo characterization of the recombinant iPEDVPT-P5, iPEDVPT-P5-96S, iPEDVPT-P96-5S and iPEDVPT-P96 viruses in conventional seven-day-old piglets. (A) Mean value of RNA copies in feces in conventional seven-day-old piglets after inoculation with recombinant viruses or PI-medium through the study. The dash line indicated the detection limit of the real-time PCR (log4.8 RNA copies per mL). Error bars represented standard deviation. (B) Mean value of fecal consistency graded daily in piglets after inoculation with recombinant viruses or PI-medium through the study. Error bars represented standard deviation. (C) Survival curve of piglets after inoculation with recombinant viruses or PI-medium through the study. (D) Mean value of average daily weight gain in different treated groups. Calculation of average daily weight gain was only performed on piglets that were not euthanized. Below is the formula used for calculation: Weight gained surviving period. Error bars represented standard deviation. Mock: PI-medium; iP5: iPEDVPT-P5; 96S: iPEDVPT-P5-96S; 5S, iPEDVPT-P96-5S; iP96, iPEDVPT-P96.