All experiments were performed in adherence to the European Directive for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (86/609/EU). The protocols were approved by the Committee for Experiments on Animals of the Faculty of Medicine, Charles University, in Pilsen and by the Ministry of Education, Youth and Sports of the Czech Republic (protocol no. MSMT-20064/2015-3). All experiments were performed in the Laboratory of Experimental Intensive Care Medicine of the Biomedical Center at the Faculty of Medicine in Pilsen. Thirty-two domestic pigs (breed Black Pied Prestice Pig) from conventional breeding facility (ZD Mladotice, Czech Republic) of either sex with a median weight of 43.5 (40–46) kg were used.