PMC:7019005 / 37222-38048 JSONTXT

{"project":"TEST0","denotations":[{"id":"32117276-222-228-3576171","span":{"begin":222,"end":224},"obj":"[\"19098906\"]"},{"id":"32117276-226-232-3576172","span":{"begin":226,"end":228},"obj":"[\"20558630\"]"},{"id":"32117276-230-236-3576173","span":{"begin":230,"end":232},"obj":"[\"22427530\"]"},{"id":"32117276-95-101-3576174","span":{"begin":330,"end":332},"obj":"[\"16923606\"]"},{"id":"32117276-221-227-3576175","span":{"begin":456,"end":458},"obj":"[\"27059199\"]"},{"id":"32117276-107-113-3576176","span":{"begin":568,"end":570},"obj":"[\"23602578\"]"}],"text":"The manufacturing of MSCs for this study was consistent with procedures applied elsewhere using fetal bovine serum for MSC propagation, trypsin for MSC harvesting, and a single freeze/thaw cycle for MSCs cryopreservation (10, 11, 15). Transplanted MSCs conformed to the criteria of the International Society for Cellular Therapy (31) and were kept for the minimum number of passages (up to four) to maintain their stem cell phenotype and avoid senescence (32). Paradoxically, the inconsistency in MSCs potency could lie in their sensitivity to a cytokine environment (33). As each pathology and even patient serum is accompanied by specific qualitative and quantitative cytokine composition, the immunomodulatory function of MSCs can be highly dependent on a particular patient's response to a specific pathology or treatment."}

{"project":"2_test","denotations":[{"id":"32117276-19098906-35127778","span":{"begin":222,"end":224},"obj":"19098906"},{"id":"32117276-20558630-35127779","span":{"begin":226,"end":228},"obj":"20558630"},{"id":"32117276-22427530-35127780","span":{"begin":230,"end":232},"obj":"22427530"},{"id":"32117276-16923606-35127781","span":{"begin":330,"end":332},"obj":"16923606"},{"id":"32117276-27059199-35127782","span":{"begin":456,"end":458},"obj":"27059199"},{"id":"32117276-23602578-35127783","span":{"begin":568,"end":570},"obj":"23602578"}],"text":"The manufacturing of MSCs for this study was consistent with procedures applied elsewhere using fetal bovine serum for MSC propagation, trypsin for MSC harvesting, and a single freeze/thaw cycle for MSCs cryopreservation (10, 11, 15). Transplanted MSCs conformed to the criteria of the International Society for Cellular Therapy (31) and were kept for the minimum number of passages (up to four) to maintain their stem cell phenotype and avoid senescence (32). Paradoxically, the inconsistency in MSCs potency could lie in their sensitivity to a cytokine environment (33). As each pathology and even patient serum is accompanied by specific qualitative and quantitative cytokine composition, the immunomodulatory function of MSCs can be highly dependent on a particular patient's response to a specific pathology or treatment."}

{"project":"MyTest","denotations":[{"id":"32117276-19098906-35127778","span":{"begin":222,"end":224},"obj":"19098906"},{"id":"32117276-20558630-35127779","span":{"begin":226,"end":228},"obj":"20558630"},{"id":"32117276-22427530-35127780","span":{"begin":230,"end":232},"obj":"22427530"},{"id":"32117276-16923606-35127781","span":{"begin":330,"end":332},"obj":"16923606"},{"id":"32117276-27059199-35127782","span":{"begin":456,"end":458},"obj":"27059199"},{"id":"32117276-23602578-35127783","span":{"begin":568,"end":570},"obj":"23602578"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"The manufacturing of MSCs for this study was consistent with procedures applied elsewhere using fetal bovine serum for MSC propagation, trypsin for MSC harvesting, and a single freeze/thaw cycle for MSCs cryopreservation (10, 11, 15). Transplanted MSCs conformed to the criteria of the International Society for Cellular Therapy (31) and were kept for the minimum number of passages (up to four) to maintain their stem cell phenotype and avoid senescence (32). Paradoxically, the inconsistency in MSCs potency could lie in their sensitivity to a cytokine environment (33). As each pathology and even patient serum is accompanied by specific qualitative and quantitative cytokine composition, the immunomodulatory function of MSCs can be highly dependent on a particular patient's response to a specific pathology or treatment."}