Figure 1A shows that 15 patients (56%) had a positive BAT with at least one mold extract: 10 BAT Af, 6 BAT Pen, and 4 BAT Alt positive. Three patients and one patient had a positive BAT with two or all the three extracts, respectively. BAT showed low levels of activation in controls, in Af-sensitized patients, and in mold-colonized patients. The ABPA group, in which no patient was not lung transplanted, responded with higher basophil activation to Af as compared to other groups, but statistical significance was not reached due to the small sample size (three patients, p = 0.066). Af-induced basophil activation in these patients was higher than responses to Pen and Alt (mean 18.48, range 17.00–21.58 for Af vs. 1.02, range 0.54–1.69 for Pen and 0.97, range 0.60–1.21 for Alt), although the significance level was not reached either (p = 0.066). These patients had no positive responses with Pen and Alt extracts. When specific IgE to Af was positive (>0.10 kUA/L), BAT Af was significantly higher than BAT Pen and Alt (p = 0.008).