Table 2  Overview of reports on cellular assays as a diagnostic tool for allergic mycosis.
References   Population   Allergen(s)   Markers   Main conclusions
Hollmann et al. (19)  79 C. albicans-colonized patients 30 healthy controls   C. albicans  LST with 3H-thymidine  57% of C. albicans-colonized patients show lymphocyte response
Brunet et al. (20)  60 C. albicans-delayed HS patients   C. albicans  LST with CD25 and CD69  All the patients have CD25 T cells Only patients with syndromic reactions have CD69 T cells
Tsushima et al. (21)  7 patients   L. aggregatum  LST with 3H-thymidine  LST is positive in peripheral blood and BAL for all patients Diagnosis of HS pneumonitis induced by L. aggregatum
Yoshikawa et al. (22)  One case report   P. citrinum  LST with 3H-thymidine  LST is positive in peripheral blood and BAL Diagnosis of HS pneumonitis induced by P. citrinum
Matsuno et al. (23)  One case report  A. alternata A. fumigatus C. albicans  LST with 3H-thymidine Evaluation of IL-5 production  LST are positive with A. fumigatus and C. albicans Only C. albicans culture produce IL-5 in vitro Diagnosis of acute eosinophilic pneumonia caused by C. albicans
Luong et al. (24)  10 allergic fungal rhinosinusitis 11 healthy controls  A. alternata A. fumigatus C. herbarum P. notatum  LST with evaluation of cytokine production after 72 h of incubation  Fungal antigens stimulate T-cell activation, with a Th2 immune response (IL-4 and IL-5 production)
Ogawa et al. (25)  Two case reports   S. commune  LST with 3H-thymidine  LST is positive in peripheral blood Diagnosis between Schizophyllum-asthma and ABPM
Mirkovic et al. (12)  48 CF patients 11 healthy controls   A. fumigatus  BAT with CD203c  BAT discriminates non-sensitized and Af-sensitized patients BAT is inversely correlated with FEV1 Antifungal therapy does not altered BAT results
Gernez et al. (11)  74 CF patients 2 asthmatic patients  A. fumigatus Asp f 1  BAT with CD203c and CD63  CD203c shows better discriminated performance than CD63 BAT with Af discriminates ABPA and no-ABPA CF patients BAT with Af does not discriminate non-sensitized and Af-sensitized patients BAT with Asp f 1 discriminates ABPA and no-ABPA CF patients
Katelari et al. (13)  56 CF patients  A. alternata A. fumigatus  BAT with CD203c and CD63  BAT with Af discriminates ABPA and no-ABPA CF patients with a high threshold BAT with Af discriminates Af-sensitized patients who run a higher risk of ABPA No correlation between BAT with Af and BAT with A. alternata
A. alternata, Alternaria alternata; ABPM, Allergic bronchopulmonary mycosis; B. adusta, Bjerkandera adusta; BAL, Bronchoalveolar lavage; C. albicans, Candida albicans; C. herbarium, Cladosporium herbarum; CF, Cystic fibrosis; FEV1, Forced expiratory volume in 1 s; HS, Hypersensitivity; L. aggregatum, Lyophyllum aggregatum; P. citrinum, Penicillium citrinum; P. notatum, Penicillium notatum; S. commune, Schizophyllum commune.