The appearance of potentially proepileptogenic changes among patches of cortical astrocytes is consistent with the hypothesis that these changes may play a causal role in epileptogenesis. Also consistent with this interpretation, changes appeared within 1 week; before the onset of spontaneous seizures. Furthermore, the size of the cortical area in which Glt1 immunoreactivity was reduced positively correlated with seizure occurrence. Nonetheless, findings in the model are correlative. Future interventional studies are needed to determine whether the astrocytic changes are cause, effect, or epiphenomena. It is also important to further characterize this model for other putative pro-epileptogenic changes, such as circuit reorganization and changes in the expression of neuronal proteins. Assessment of neuronal function in the astrocytic patches exhibiting protein downregulation relative to cortical regions containing apparently healthy astrocytes will be of particular interest. The abnormal patches are predicted to exhibit potassium accumulation, glutamate accumulation, and localized hyperexcitability.