H441 cells and human bronchial epithelial cells (HBECs) express all three forms of hexokinase. Representative Western blots of cell lysates from H441 cells grown on plastic (A) or H441 cells or HBECs grown at air-liquid interface (B). Lanes indicate cell type and growth conditions of either 5 or 15 mM glucose as indicated. Proteins immunostained for hexokinases I, II, and III are indicated to the right of the blots (all ~100 kDa). The immunostained housekeeping protein β-actin is also indicated (Actin) and serves as a loading control.