Figure 2  Three yellow cis‐regulatory elements (CREs) encode tissue‐specific expression in Bombyx mori. (A) Genome structure around the yellow gene. For CRE analyses, the 22‐kb yellow locus was dissected into six fragments (I–VI). Top bars indicate the six DNA fragments screened for using reporter assays in embryonic larvae. The blue bar represents the thoracic leg CRE (fragment III), the turquoise bar marks the location of the hair CRE (fragment V) and the red bar exhibits the trachea CRE (fragment VI). At the bottom, the black bar represents the basal promoter for the reporter assay and the triangle represents the translation start site, ATG. In transgenic B. mori embryonic larvae, green fluorescent protein reporter protein expression was driven by the thoracic leg CRE (B), the hair CRE (C) and the trachea CRE (D). Scale bars = 500 µm.