2.2. Phage Display Random Peptide Libraries
Two phage display random peptide libraries were previously constructed on vector fUSE55 displaying inserts X6 or CX8C (C indicates cysteine; X indicates any amino acid) that were fused to an outer minor phage coat protein (pIII) at the N-terminus of an Fd phage [24,25]. These tetracycline-resistant libraries consisted of a linear, 6-peptide configuration (X6), or a cyclic, 8-peptide configuration (CX8C) with a loop scaffold developed by flanking the random peptide sequence with a pair of cysteine residues to form a bridge. The details of biopanning, binding assays, and ELISA procedures with synthetic peptides are described in the Supplementary Materials (A. Phage Display).