Functional categorization of proteome at planktonic and biofilm stages

KEGG pathways
The proteins identified in the planktonic and biofilm stages were assigned to define KEGG pathways. The major pathways were associated with ribosome (72 hits), pyruvate metabolism (27 hits), pyrimidine metabolism (50 hits), amino acid biosynthesis (69 hits) and antibiotics (122 hits), Fatty acid metabolism (15 hits), RNA degradation (13 hits), biosynthesis of secondary metabolites (157 hits), glycolysis/gluconeogenesis (40 hits), citrate cycle (TCA cycle) (7 hits), microbial metabolism in diverse environments (110 hits), propanoate metabolism (11 hits) and butanoate metabolism (9 hits). Furthermore, the proteins upregulated/unique to biofilm stages were assigned to KEGG pathways and revealed the enrichment of glycolysis/gluconeogenesis, pyruvate metabolism, biosynthesis of secondary metabolites, microbial metabolism in diverse environments and arginine metabolism (Fig. 2). More than 50% of the total proteins involved in these pathways were detected to be upregulated in biofilm stages of SK460.
Fig. 2 KEGG pathways and molecular functions assigned to categorize the upregulated proteins obtained in biofilm stages of E. faecalis SK460