Peptide samples were analyzed by nano-LC/MSE (at elevated energy) using a nanoACQUITY UPLC® system (Waters, Altrincham, UK) coupled to a Quadrupole-Time of Flight (Q/TOF) mass spectrometer (SYNAPT-G2, Waters, Altrincham, UK). Instrument operation and control for both the system was done employing MassLynx4.1 SCN781 software. The peptides were separated by reverse-phase column chromatography in the nanoACQUITY UPLC®. Peptides eluted from the nano-LC were subjected to mass spectrometric analysis on a SYNAPT® G2 High Definition MS™ System (Waters, Altrincham, UK). All analyses were performed using positive mode ESI using a NanoLockSpray™source. The detailed operational set up was followed as in previous publications [44].